Tissue engineering: the study the bioprocessing of human and mammalian stem cells in 3D dynamic environments to mimic and develop neotissue constructs, the design of medical devices and clinical scale bioreactors.

Undergraduate students

2025-present Valentina Parra, Biotechnology engineering, Chemical Engineering Department, Universidad de La Frontera, Chile.
2025-present Maria Jesus Alarcon, Biotechnology engineering, Chemical Engineering Department, Universidad de La Frontera, Chile.
2024 Camila Antilao, Biotechnology engineering, Chemical Engineering Department, Universidad de La Frontera, Chile.
2024 Francisco Farias, Design Of 3D-Printed Biological Scaffold Structure For Application In Bone Implants Validated In PLA, Mechanical Engineering Department, Universidad de Talca, Chile.
2024 Isaias Colihuil, Biotechnology Engineering, Chemical Engineering Department, Universidad de La Frontera, Chile.
2022 Nicolas Lefín, Chemical Engineering, Chemical Engineering Department, Universidad de La Frontera, Chile.
2022 Javiera Miranda, Biotechnology Engineering, Chemical Engineering Department, Universidad de La Frontera, Chile.
2016-2017 Stefania Aracena, Biotechnology Engineering, Chemical Engineering Department, Universidad de La Frontera, Chile.

Graduate students

2025-present Loraine Cotes, PhD program in Sciences with specialization in Applied Cellular and Molecular Biology, Universidad de La Frontera, Chile.
2025-present Daniel Ulloa, PhD program in Sciences with specialization in Applied Cellular and Molecular Biology, Universidad de La Frontera, Chile.
2024-present Rodrigo Irarrázaval, PhD program in Engineering of MacroFacultad Consortium, UFRO-UTalca-UBB, Chile.
2024-present Dayana Perez, PhD program in Sciences with specialization in Applied Cellular and Molecular Biology, Universidad de La Frontera, Chile.
2024-present Aloyma Lugo, PhD program in Sciences with specialization in Applied Cellular and Molecular Biology, Universidad de La Frontera, Chile.
2024-present Luis Jimenez, PhD program in Sciences with specialization in Applied Cellular and Molecular Biology, Universidad de La Frontera, Chile.
2022-2023 Nolberto Huard, MSc program in Quality Management Systems, Universidad de La Frontera, Chile.

2025 IV National Congress of Tissue Engineering and Regenerative Medicine, ITMR 2025, Pucón, Chile, to be held 13-14/11/2025 (part of scientific comity).
2024 M. Zamorano. “La ingeniería de tejidos y su potencial en modelos no animales de enfermedades”, Workshop "BIOSMART: Biomateriales Inteligentes para la Ingeniería de Tejidos", Universidad Adolfo Ibáñez, Viña del Mar, Chile, 9-10/01/2025.
2023 M. Zamorano, “Tissue engineering principia and its applications in Pharmaceutical Science”, Academic Lecture at the Faculty of Pharmaceutical Sciences, Universidade de Sao Paulo, São Paulo, Brazil. 31/08/2023.
2023 N. Lefin, J. Miranda, G. Monteiro, I. Munhoz, M. Zamorano, A. Pessoa Jr., JG. Farias. “Amino acid addition to improve chemically defined medium for production of a double mutant l-asparaginase ii from erwinia chrysantemi expressed by escherichia coli bl21 (de3)”, XLV Congreso Chileno de Microbiología, Pucón, Chile, 4-7/12/2023.
2023 J. Miranda, N. Lefin, I. Munhoz, M. Zamorano, G. Monteiro, A. Pessoa Jr., JG. Farias. “Optimization of culture medium for extracellular production of L-asparaginase in Escherichia coli BL21 (DE3)”, XLV Congreso Chileno de Microbiología, Pucon, Chile, 4-7/12/2023.
2023 N. Lefin, J. Miranda, G. Monteiro, I. Munhoz, M. Zamorano, A. Pessoa Jr., JG. Farias. “Sumplementação de aminoácidos para a obtenção de um meio quimicamente definido aprimorado para a produção de uma l-asparaginase ii dupla mutante expressa por escherichia coli bl21 (de3).”, 32° Congresso Brasileiro de Microbiología, Pucon, Chile, 18-22/10/2023.
2023 J. Miranda, N. Lefin, IM Costa, G Monteiro, M. Zamorano, A. Pessoa Jr, JG. Farias; “Otimização do meio de cultura para a produção de l-asparaginase extracelular em escherichia coli bl21 (de3)”, 32° Congresso Brasileiro de Microbiología, Pucon, Chile, 18-22/10/2023.
2020 M. Zamorano. “Tissue engineering: solving the human regeneration jigsaw”, Training cycle for engineering students, AetiqBA, Buenos Aires, Argentina, 12/10/2020 (Speaker).
2020 M. Zamorano. “Tissue engineering: solving the human regeneration jigsaw”, Chemical Engineering Student Association Talks, AChEIQ, Santiago, Chile, 10/08/2020 (Speaker).
2018 M. Zamorano. “Tissue engineering & bone reconstruction”, National Congress of engineering students in biotechnology, CONEIB, Temuco, Chile, 23/10/2018 (Keynote speaker and part of scientific comity).
2014 London International Youth Science Forum (LIYSF), laboratory tutor for “The Blood factory Project”, Imperial College London, UK, 23/7-6/8, 2014.
2014 Chileglobal seminars London: part of the organisers and chair for “Biology, Biotechnology and Natural Sciences seminar”, University College London, UK, 20/02/2014.
2014 Imperial College Flow Cytometry course, Faculty of Medicine, ICL, UK, 4-5/9, 2014.
2012 Mahn, A.; Zamorano, M.; Cartes, P.; Reyes, A., “Effect of freeze-drying conditions on antioxidant compounds of brócoli”, 2012 EFFoST Annual Meeting, 20 – 23/11, 2012, Montpellier, France.
2012 Mahn, A.; Reyes, A.; Antoniz, D.; Zamorano, M., Pinto, J., “Evaluation of different drying systems to obtain a broccoli-based funtional ingredient”, 2012 EFFoST Annual Meeting, 20 – 23/11, 2012, Montpellier, France.
2011 A. Mahn, M. Zamorano, W. Cifuentes, M. Ismail, “Depletion of highly-abundant proteins in blood plasma by hydrophobicity-based separation techniques”, 16th Internacional Conference on Biopartitioning and Purification - BPP2011, 18-22/09, 2011, Puerto Vallarta, Mexico.
2011 A. Mahn; A. Reyes; M. Zamorano; P. Antoine; C. Guzman, “Effect of dehydration conditions on the content to antioxidant and anticarcinogenic compounds in brócoli”, XXV Interamerican Congress of Chemical Engineering, 14 – 17/11, 2011, Santiago, Chile.
2011 M. Zamorano, A. Reyes and A. Mahn (2011). “Effect of Freeze-drying Conditions on Antioxidant Properties of Se-enriched Broccoli”, XXV Interamerican Congress of CE & XVIII Chilean Congress of CE, 14-16/11, 2011 (oral presentation).
2011 A.Mahn; M. Zamorano, A. Reyes, “Optimization of the freeze-drying of broccoli florets”, XXXV Congresso Brasileiro de Sistemas Particulados, 23 – 16/10, 2011, Vassouras, Brazil.
2009 Proteomic Seminar, Universidad de Chile, 13-17/4, 2009.
2009 M. Zamorano, F. Cubillos, A. Mahn, "Applicability of the theoretical dish model to the simulation of hydrophobic interaction chromatography (HIC)", XVII Chilean Congress of Chemical Engineering, 25 – 28/10, 2009, Viña del Mar, Chile.
2008 A. Mahn and M. Zamorano (2008)."Depletion of highly abundant proteins in blood plasma by hydrophobic interaction chromatography for proteomic analysis", A. Mahn y M. Zamorano. COLACRO XII Latin American Congress of Chromatography and related Techniques, 28-30/10, 2008, Florianapolis, Brazil. (oral presentation).
2008 M. Zamorano and A. Mahn. "Depletion of highly abundant proteins in rat blood plasma by hydrophobic chromatography for proteomic analysis", LI Meeting Society of Biology of Chile, 26 – 29/10, 2008, Pucón, Chile.